Short-term preservation of sperm under oxygen atmosphere in rainbow-trout (salmo-gairdneri)
1981
Billard, Roland | Laboratoire de physiologie des poissons ; Institut National de la Recherche Agronomique (INRA)
Experiments were carried out to test the possibilities of using plastic bags inflated with oxygen to preserve and to transport rainbow trout sperm. Sperm survival is improved after storage in an O2 atmosphere in comparison with storage in air. After storage for 5 days (Exp. 1) fertilizing ability was similar under O2 to that of the controls (freshly collected sperm) but under air it was significantly lower (P < 0.001). In another experiment (Exp. 2), fertilizing capacity depended on the rate of dilution and the quality of the eggs. With some females high levels of fertilization were recorded after 14 days of sperm storage and with other females fertilizing capacity of the same sperm drops within one day. This variability in the response still limits the practical application of short-term preservation of rainbow trout sperm.
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