Biological control of bacterial wilt in Arabidopsis thaliana involves abscissic acid signalling
2012
Feng, Dong Xin | Tasset, Celine | Hanemian, Mathieu | Barlet, Xavier, X. | Hu, Jian | Tremousaygue, Dominique | Deslandes, Laurent | Marco, Yves, Y. | Laboratoire des interactions plantes micro-organismes (LIPM) ; Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS) | UMR 2594 ; Centre National de la Recherche Scientifique (CNRS) | Chinese Academy of Agricultural Sciences (CAAS) | Unité mixte de recherche interactions plantes-microorganismes ; Institut National de la Recherche Agronomique (INRA)-Université Toulouse III - Paul Sabatier (UT3) ; Université de Toulouse (UT)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS) | Coll Biol Sci ; China Agricultural University (CAU) | National Science Foundation of China [31071675, 30671179]; French Ministry for Higher Education and Research CNRS; Agence Nationale de la Recherche [PCS-08-GENO-SCRIPS]
International audience
Mostrar más [+] Menos [-]Inglés. Means to control bacterial wilt caused by the phytopathogenic root bacteria Ralstonia solanacearum are limited. Mutants in a large cluster of genes (hrp) involved in the pathogenicity of R similar to solanacearum were successfully used in a previous study as endophytic biocontrol agents in challenge inoculation experiments on tomato. However, the molecular mechanisms controlling this resistance remained unknown. We developed a protection assay using Arabidopsis thaliana as a model plant and analyzed the events underlying the biological control by genetic, transcriptomic and molecular approaches. High protection rates associated with a significant decrease in the multiplication of R similar to solanacearum were observed in plants pre-inoculated with a similar to hrpB mutant strain. Neither salicylic acid, nor jasmonic acid/ethylene played a role in the establishment of this resistance. Microarray analysis showed that 26% of the up-regulated genes in protected plants are involved in the biosynthesis and signalling of abscissic acid (ABA). In addition 21% of these genes are constitutively expressed in the irregular xylem cellulose synthase mutants (irx), which present a high level of resistance to R similar to solanacearum. We propose that inoculation with the ?hrpB mutant strain generates a hostile environment for subsequent plant colonization by a virulent strain of R similar to solanacearum.
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