Evaluation of anti-oxidant activities and total phenolic content of Chromolaena odorata
2010
Srinivasa Rau, K. | Chaudhury, Pradeep Kumar | Pradhan, Anshuman
The aim of this study was to assess the in vitro potential of chloroform extract of Chromolaena odorata leaves. The DPPH activity of the extract (0.1–5 mg/ml) was increased in a dose dependent manner, which was found in the range of 23.48–91.61% as compared to ascorbic acid (33.69–94.10%). The IC50 values of chloroform extract in DPPH radical, hydroxyl radical, nitric oxide, ABTS radical were obtained to be 0.31, 0.43, 0.28 and 1.32 mg/ml, respectively. However, the IC50 values for the standard ascorbic acid were noted to be 0.24, 0.41, 0.23 and 1 mg/ml, respectively. Measurement of total phenolic content of the chloroform extract of C. odorata was achieved using Folin–Ciocalteau reagent containing 242.2 mg/g of phenolic content, which was found significantly higher when compared to reference standard gallic acid. The results obtained in this study clearly indicate that C. odorata has a significant potential to use as a natural anti-oxidant agent.
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