Variation in chromosome numbers and nuclear DNA contents in genetic resources of Lactuca L. species (Asteraceae)
2002
Dolezalova, I. | Lebeda, A. | Janecek, J. | Cihalikova, J. | Kristkova, E. | Vranova, O.
Fifty accessions of 25 Lactuca species, L. serriola x L. sativa and Mycelis muralis were analyzed for chromosome number and relative DNA amount variation. In the majority of Lactuca species studied chromosome counts, as earlier reported (n = 8, 9, 17), were verified; however, for L. dregeana and L. homblei (probably L. schweinfurthii or L. longespicata) the chromosome number (n = 9) was determined for the first time. Relative nuclear DNA content, estimated by using flow cytometry (DAPI staining), showed that 2C DNA content ranged from 2.02 pg in L. capensis to 17.96 pg in L. canadensis. Statistical and cluster analysis of data based on relative nuclear DNA contents correspond fairly well with recently accepted taxonomic classification of the genus Lactuca. However, the position of certain species as well as clarification of taxonomic determinations of some Lactuca accessions needs further examination.
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