Localization of integrin heterodimer α₉β₁ on the surface of uterine endometrial stromal and epithelial cells in mice
2020
Park, Hye Jin | Yun, Jung Im | Lee, Seung Tae
Previously, we reported that endometrial stromal (ES) and endometrial epithelial (EE) cells did not attach to tenascin C, indicating the absence of active integrin α₉β₁ on the surface of mouse ES and EE cells. However, that study used recombinant tenascin C without fibronectin (FN) type III repeats interacting with integrin heterodimers. Therefore, we re-evaluated the presence of integrin α₉β₁ actively functioning on the surface of mouse ES and EE cells using full-length native tenascin C with FN type III repeats. The functionality of integrin α₉β₁ was confirmed using attachment and antibody inhibition assays. Both mouse ES and EE cells showed significantly increased adhesion to native tenascin C, and functional blocking of integrin α₉β₁ significantly inhibited adhesion to native tenascin C. These results demonstrate that the integrin α₉ and β₁ subunits function as active heterodimers on the plasma membrane of mouse ES and EE cells, respectively.
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