An efficient protocol for the production of triploid plants from endosperm callus of neem, Azadirachta indica A. Juss
2003
Chaturvedi, R. | Razdan, M.K. | Bhojwani, S.S.
Triploid plants of neem were obtained by immature endosperm culture. Immature seeds, at the early dicotyledonous stage of embryo development, is the best explant to raise endosperm callus on MS + NAA (5 micromol/L) + BAP (2 micromol/L) + CH (500 mg L(-1)). Maximum shoot bud differentiation from the endosperm callus occurred on MS + 5 micromol/L BAP. Shoots were multiplied by forced axillary branching and rooted in vitro. The plants were established in soil. Over 66% of the plants were triploid with chromosome number 2n = 3x = 36. A characteristic feature of the shoots of endosperm origin is the presence of a large number of multi-cellular glands.
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