Biosurfactant production from Candida lipolytica in bioreactor and evaluation of its toxicity for application as a bioremediation agent
2017
Santos, Danyelle Khadydja F. | Meira, Hugo M. | Rufino, Raquel D. | Luna, Juliana M. | Sarubbo, Leonie A.
This large-scale production, toxicity, characterization and economic analysis of the biosurfactant from Candida lipolytica UCP 0988 produced in the low-medium formulated with animal fat and corn steep liquor was investigated. The biosurfactant was produced in the stationary phase under 200rpm in the absence of aeration and reduced the surface tension of the medium from 50 to 28mN/m after 96h, yielding 10.0g/L of isolated biosurfactant in a 2L bioreactor. The production was maximized in a 50L bioreactor, reaching 40g/L biosurfactant and 25mN/m. The cell biomass was quantified and characterized for use in animal nutrition. Chemical structures of the biosurfactant were identified using FTIR and NMR. The crude biosurfactant was not toxic to the bivalve Anomalocardia brasiliana, to the microcrustacean Artemia salina, or three species of vegetables seeds. The biosurfactant stimulated the degradation of motor oil by the seawater indigenous microorganisms. The results obtained indicate that the biosurfactant produced has great potential to be applied as a bioremediation agent for cleaning oil spills.
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