Production of somatic hybrids between Brassica oleracea and the C3-C4 intermediate species Moricandia nitens
1999
Yan, Z. | Tian, Z. | Huang, B. | Huang, R. | Meng, J.
Protoplast fusion between Brassica oleracea and Moricandia nitens, C(3)-C(4) intermediate wild species, was carried out. Four hundred and twenty five plants were regenerated from 1995 calli. More than 90% of the regenerated plants were verified as true intergeneric hybrids on the basis of morphological observation and molecular-marker analysis. The hybrids were morphologically intermediate between both fusion parents. Variations in flower color and petal number were also observed. The chromosome number and pollen fertility varied across the individual hybrids. Although after self-pollination pollen germinated on the stigma and pollen tubes were visible in the style, the pods did not develop properly without in vitro culture. Measurements of the CO(2) compensation point revealed that six out of eight hybrid plants expressed a gas-exchange character that was intermediate between the C(3)-C(4) M. nitens and C(3) B. oleracea parents.
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