Chemical composition, cytotoxic effect and antimicrobial activity of Stachys koelzii Rech.f. essential oil against periodontal pathogen Prevotella intermedia
2018
Ramak, Parvin | Talei, Gholam Reza
Prevotella intermedia is associated with periodontal diseases and endodontic infections. Periodontitis can be suppressed by utilizing the antiseptics, which target the infectious bacteria. The member of Stachys sp. has been used traditionally in the form of decoction or infusion for management of infectious diseases. The subject of this article was to evaluate the chemical composition, antimicrobial and cytotoxic effect of Stachys koelzii essential oil and its main components against Prevotella intermedia. GC-FID and GC-MS analysis were used to determine the chemical composition. The antimicrobial effects of S. koelzii essential oil was evaluated by micro-broth dilution assay. Time kill curve assays, leakage of cytoplasmic materials and anti-biofilm effects were determined. Its cytotoxic effect was evaluated by MTT assay. Essential oil with main components of α-pinene, trans-caryophyllene and 1,8-cineole inhibited P. intermedia with MIC and MBC values of 0.1 and 0.2 mg/mL. Its biofilm formation was higher than α-pinene, followed by trans-caryophyllene and 1,8-cineole. Essential oil and its main components increased the leakage of cytoplasmic components. Essential oil showed cytotoxic effect on HeLa cell lines with IC50 0.06 mg/mL. The cytotoxic effect of α-pinene on healthy cell lines was higher than essential oil. S. koelzii essential oil can be used in mouthwash formulations and its efficacy should be evaluated in large clinical studies.
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