Structure of a lytic polysaccharide monooxygenase from Aspergillus fumigatus and an engineered thermostable variant
2018
Lo Leggio, Leila | Weihe, Cecilia D. | Poulsen, Jens-Christian N. | Sweeney, Matt | Rasmussen, Frank | Lin, Janine | De Maria, Leonardo | Wogulis, Mark
Lytic polysaccharide monooxygenases (LPMOs) are industrial enzymes which are gaining use in second generation bioethanol production from lignocellulose by acting in synergy with glycoside hydrolases. Here we present the X-ray crystal structure of an AA9 fungal LPMO from Aspergillus fumigatus and a variant which has been shown to have better performance at elevated temperatures. Based on the structures, thermal denaturation data and theoretical calculations, we provide a suggestion for the structural basis of the improved stability.
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