Determination of total avermectin B1 and 8,9-Z-avermectin B1 residues in wine by liquid chromatography
1996
Cobin, J.A. | Johnson, N.A.
A liquid chromatographic method was developed and validated for determination of avermectin B1 and 8,9-Z-avermectin B1 residues in wine. The sample is extracted with hexane acetonitrile and the hexane layer containing the avermectins is concentrated/purified on an aminopropyl solid-phase extraction (SPE) column. The purified extract is derivatized with trifluoroacetic anhydride and the derivatized avermectins are analyzed by reversed phase liquid chromatography with fluorescence detection. Recoveries of avermectins from wine fortified with approximately 1-25 ng/g avermectin B1a or 8, 9-Z-avermectin B1a averaged 88 and 102%, respectively. The limit of quantitation is 1 ng/g (signal-to-noise ratio [S/N] > 10) and the limit of detection is 0.5 ng/g (S/N > 3) for each analyte. This procedure provides a simple, rapid, and sensitive method for monitoring the total amount of avermectin residues in wine.
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