Lipid, protein and fatty acid profiles of Emericella sp. in different media
2010
Verma, Shikha | De, Bijay Krishna
The soil-derived fungus Emericella sp. was explored for its potential to produce lipid. Lipid profile, fatty acid composition, production of proteins and carbohydrates from lipid-extracted biomass were determined. The effects of variations in the contents of carbon sources (glucose, dextrose), the salt content (NaCl) and the growth period were studied. A study on the effect of different growing media on the above-mentioned parameters was also carried out. Although the maximum amount of lipid (6.14 ± 0.42 g/L) and protein (5.99 ± 0.47 g/L) was produced after 13 days in medium A containing 10% wt/vol glucose, the optimum lipid (2.90 ± 0.21 g/L) and protein (3.23 ± 0.28 g/L) production was observed in 2% wt/vol glucose medium considering the glucose content in the medium. The principal fatty acids found were 16:0 (14.4 ± 1.0 to 24.5 ± 2.4 wt-%), 18:0 (12.1 ± 0.4 to 27.7 ± 2.7 wt-%), 18:1 (13.5 ± 2.1 to 25.2 ± 2.8wt-%) and 18:2 (30.9 ± 2.0 to 47.0 ± 2.8 wt-%). Some of the lipids, especially those grown for 7 days in less glucose-containing (1 and 2% wt/vol) medium were found to contain nearly 9.0 wt-% of long-chain PUFA 18:4, 20:4, 20:5, 22:4, and 22:5).
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