Study of the bio-production of carotenoids by Sporidiobolus salmonicolor (CBS 2636) using pre-treated agro-industrial substrates
2008
Valduga, Eunice | Valério, Alexsandra | Treichel, Helen | Di Luccio, Marco | Furigo Júnior, Agenor
BACKGROUND: The increasing industrial demand for carotenoids has aroused interest in their bio-production, and the need to reduce production costs has encouraged the use of low cost industrial substrates, such as agro-industrial residues. Thus the objective of this research was the bio-production of carotenoids by Sporidiobolus salmonicolor using agro-industrial substrates (corn steep liquor and sugarcane molasses), pre-treated with acids (sulphuric and phosphoric).RESULTS: Bio-production was carried out in an orbital shaker using a 10% (v/v) inoculum, incubation at 25 °C, and agitation at 180 rpm for 120 h in a non-illuminated environment. The carotenoids were recovered using liquid N₂ combined with dimethylsulphoxide for cell rupture, and an acetone/methanol mixture (7:3 v/v) for extraction.CONCLUSION: The complete second-order design allowed for optimisation of the carotenoid concentration obtained from industrial substrates pre-treated with acids (sulphuric and phosphoric), obtaining a total carotenoid content of 541.5 μg L⁻¹ using 10 g L⁻¹ sugarcane molasses, 5 g L⁻¹ corn steep liquor and 5 g L⁻¹ yeast hydrolysate at 25 °C, with agitation at 180 rpm and an initial pH of 4.0.
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