Efficient itaconic acid production by Aspergillus terreus –Overcoming the strong inhibitory effect of Manganese
2019
Saha, Badal C. | Kennedy, Gregory J.
Itaconic acid (IA), a building block platform chemical, is produced industrially by Aspergillus terreus utilizing glucose. Expanding the use of IA requires lowering its production cost. Lignocellulosic biomass has the potential to serve as a low cost source of sugars for IA production. However, the fungus could not produce IA from dilute acid pretreated and enzymatically saccharified wheat straw hydrolyzate even at 100 fold dilution. Furfural, hydroxymethyl furfural and acetic acid were inhibitory, as is typical, but Mn2+ was particularly problematic for IA production. It was present in the hydrolyzate at a level that was 230 times over the inhibitory limit (50 ppb). Recently, it was found that PO4(3-) limitation decreased the inhibitory effect of Mn(2+) on IA production. In the present study, a novel medium was developed for production of IA by varying PO4(3-), Fe3+ and Cu2+ concentrations using response surfacemethodology which alleviated the strong inhibitory effect of Mn2+. The fungus was able to produce IA very well in the presence of Mn2+ up to 100 ppm in the newly developed medium. This medium will be extremely useful for IA production in the presence of Mn2+. This is the first report on the development of Mn2+ tolerant medium for IA production by A. terreus.
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