Improving L-serine production in Escherichia coli via synthetic protein scaffold of SerB, SerC, and EamA
2019
Tran, Kim-Ngan Thi | Eom, Gyeong Tae | Hong, Soon Ho
Although L-Serine has wide applications and great market potential, its application is limited by its high cost and low yields. Microbial factories have many advantages for producing L-serine from renewable carbon resources. To achieve L-serine production with high efficiency, three enzymes (SerB, SerC, and EamA) were physically re-localized by using a scaffold system GBD:SH3:PDZ. Such strategy was highly effective in improving the production of L-serine in Escherichia coli. The highest concentration of L-serine harvested was at 3.8 g/l after 48 h in strain E. coli with the scaffold GBD:SH3:PDZ at ratio of 1:2:1, reaching a high yield of 0.24 mol/mol glucose.
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