Callus induction, somatic embryogenesis and organogenesis in Narcissus confusus: correlation between the state of differentiation and the content of galanthamine and related alkaloids
1999
Selles, M. | Viladomat, F. | Bastida, J. | Codina, C.
In vitro cultures from two strains of Narcissus confusus (Amaryllidaceae) initiated from mature seeds were screened for their ability to produce alkaloids. Protocols for callus induction, somatic embryogenesis and organogenesis were established. The alkaloid contents were determined by HPLC. Undifferentiated calli produced small amounts of galanthamine, which increased with the degree of tissue differentiation. Scanning electron micrographs of the cultures at different stages of development were made. Embryogenic friable calli were maintained in culture for more than 2 years, retaining a high regeneration capability. All regenerated plants showed normal morphological characteristics.
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