Thermoregulatory effects of ergovaline isolated from endophyte-infected tall fescue seed on rats
1994
Zhang, Q. | Spiers, D.E. | Rottinghaus, G.E. | Garner, G.B.
Ergovaline (EV) was isolated from Acremonium coenophialum-infected tall fescue seed. Seed (2 kg) was extracted with 20 L of 5% lactic acid in water/methanol (4:1), filtered, and passed over an Amberlite XAD-2 column. Ergovaline was eluted with MeOH, concentrated, and chromatographed on two reversed-phase C18 columns (purity > 95%, 40% overall recovery). It was administered to rats (15 micrograms/kg of BW, ip) at 22 degrees C to determine time-related acute effects on thermoregulatory ability. Rectal temperature in EV-treated rats decreased 1.6 degrees C by 50 min (p less than equal to 0.05), due to reduction in heat production (p less than or equal to 0.05) and increase in heat loss across the tail (p less than or equal to 0.05) at 10-20 min postinjection. This study provides a rapid, economical method for milligram-scale isolation of ergovaline and defines the thermoregulatory mechanisms responsible for ergovaline-induced hypothermia under nonstress conditions.
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