Transgressive segregation for reduced glucosinolate content in Brassica carinata A. Braun
2006
Márquez-Lema, A. | Fernández-Martínez, J.M. | Pérez-Vich, B. | Velasco, L.
Successful commercial utilization of the meal by-product of Brassica oilseed crops requires the cultivation of cultivars with low glucosinolate (GSL) content in the seeds; however, such cultivars are not yet available in Brassica carinata. The objective of the present research was to search for transgressive segregants with further-reduced GSL content in the progeny of crosses involving four B. carinata lines with reduced GSL content (90 compared with 120 μmol/g seed in standard germplasm). The four lines were crossed following a diallel design and F₂ phenotypes (F₃ seed bulked) were analysed for GSL content. F₂ phenotypes with a transgressive GSL content lower than the parents were identified in all crosses involving the line S2-1241, suggesting that this line carries alleles for reduced GSL content not present in the other lines. F₃ : ₄ lines from transgressive F₂ phenotypes were evaluated for 2 years, which resulted in the selection of an F₃ : ₄ line with an average GSL content of 58 and 46 μmol/g seed, respectively compared with 84 and 62 μmol/g seed, respectively in S2-1241.
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