8-hydroxy-2'-deoxyguanosine as a biomarker for detection of the early stage induced adenocarcinoma in rats
2008
TOKARZ, ANDRZEJ | Bobrowska, Barbara | GORZATA KOSK, MAŁ
The influence of fats on carcinogenesis is associated with oxidative DNA lesions. In this study, the effect of heated (for 10 min at 200C) and unheated olive and sunflower oils on oxidative DNA damage (8-hydroxy-2'-deoxyguanosine [8-OhdG]) in rats in which breast cancer was simultaneously induced with 7,8-dimethylbenz[a]antracene (DMBA) was assessed. The research focused on the kinetics of alterations in urinary 8-OHdG at the early stage of carcinogenesis, as well as the influence of dietary factors on the process. Urine samples were obtained from animals placed individually in metabolic cages for 24 h. The study results showed that both the quantity and quality of dietary fats exert their influence on urinary 8-OHdG in rats. In addition, DMBA-induced carcinogenesis was accompanied by increased urinary 8-OHdG levels in rats, regardless of their diet. It is estimated that approximately one-third of human cancers are associated with inappropriate dietary habits and lifestyle. The aim of the present study was to assess the effect of olive and sunflower oils, heated and unheated, on the severity of oxidative DNA lesions (8-hydroxy-2'-deoxyguanosine) in rats in which breast adenocarcinoma was simultaneously induced with 7,8-dimethylbenz[a]antracene. The results presented in this work can be useful in the analysis of fats' influence on cancer process in humans.
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