High efficiency plant regeneration from petiole explants of Carica papaya L. through organogenesis
1993
Hossain, M. | Rahman, S.M. | Islam, R. | Joarder, O.I.
Callus cultures were obtained from petiole explants of Carica papaya on MS medium containing 0.5-10.5 micromolar alpha-naphthaleneacetic acid (NAA) in combination with 0.5-5 micromolar benzyladenine (BA). Hard-green calli were transferred to MS medium containing 100 mg l-1 casein hydrolysate (CH) with specific BA-NAA formulation, where they developed adventitious buds within 2 weeks of culture. Maximum number of adventitious buds were obtained in 2 micromolar BA and 0.1 micromolar NAA. Shoot regeneration occurred from these adventitious buds by the end of the 4th week. Regenerated shoots were elongated in hormone-free medium and rooted in half-strength MS fortified with 3 micromolar NAA and 0.5 micromolar gibberellic acid (GA3). The regenerants were transferred to soil after acclimatization.
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