Isolation and characterisation of phosphate solubilizing microorganisms from peat
2020
Hii, Yiik Siang | Yen San, Chan | Lau, Shiew Wei | Danquah, Michael Kobin
In acidic soil, the fixation of phosphate by aluminium and iron is very pronounced which limits the availability of soluble phosphate for plant uptake. Often, phosphatic fertilizer is added to fulfill the phosphate requirement of plants. However, frequent fertilization is unfavorable as it affects soil health and therefore, sustainable alternative caught the attention of researchers. Phosphate solubilizing microorganisms (PSMs) secrete organic acids and phosphatases which convert insoluble phosphates into soluble phosphate which can be utilized by plants. Remarkably, beneficial PSMs are generally native isolates from the location and cropping systems. Thus, current study isolated PSMs from peat in Sarawak, Malaysia. Sixteen distinct bacteria strains were isolated and the quantitative estimation of the isolated PSMs to solubilize aluminium phosphate were carried out using NBRIP broth. Three isolates with the highest aluminium phosphate solubilization potential were characterized using gram stain and 16s rRNA sequencing. These isolates were identified as Staphylococcus haemolyticus and Staphylococcus cohnii from the phylogenetic analysis.
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