Scutellaria polysaccharide inhibits the infectivity of Newcastle disease virus to chicken embryo fibroblast
2014
Xiaona, Zhao | Jianzhu, Liu
BACKGROUND: To select the antiviral active site of Scutellaria polysaccharide (SPS), safe concentrations of crude total Scutellaria polysaccharide (SPSₜ) and fractional polysaccharide SPS₅₀, SPS₆₀, SPS₇₀ and SPS₈₀ on chicken embryo fibroblast (CEF) were first compared using the MTT method. Then, SPSₜ, SPS₅₀, SPS₆₀, SPS₇₀, and SPS₈₀ at five concentrations within the safe concentration, together with Newcastle disease virus (NDV), were added to the cultivating system of CEF in three models: pre‐addition of polysaccharide, post‐addition of polysaccharide, and simultaneous addition of polysaccharides and NDV after mixing. The effects of SPS on the cellular infectivity of NDV (A₅₇₀ value and the highest viral inhibitory rate) were compared using the MTT method. RESULTS: At appropriate concentrations, the five polysaccharides could significantly inhibit the infectivity of NDV on CEF. Among the five polysaccharide groups, the SPS₈₀ group exhibited the highest viral inhibitory rate in the three sample‐addition modes. CONCLUSION: This finding indicates that SPS₈₀ possesses the best efficacy as a component of antiviral polysaccharide drug. © 2013 Society of Chemical Industry
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