A luminescence immunoassay test method for determining benzo[a]pyrene in natural water
2017
Beloglazova, N. V. | Shmelin, P. S. | Novikova, A. S. | Goryacheva, I. Yu
A test method is developed for determining benzo[a]pyrene in natural water, based on the use of a polyethylene filter (frit) with adsorbed specific antibodies, placed within a transparent column. In passing a test solution, the analyte is adsorbed on the frit similarly to the process implemented in immunoaffinity preconcentration. The added conjugate of a labeled analyte takes the remained vacant binding sites of antibodies. Luminescent semiconductor nanoparticles (quantum dots) CdSe/ZnS, used as labels, enable visual determination under irradiation with UV light. The limit of detection for benzo[a]pyrene in water is ~0.5 ng/mL.
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