Cloning of the y1 locus of maize, a gene involved in the biosynthesis of carotenoids
1990
Buckner, B. | Kelson, T.L. | Robertson, D.S.
The y1 gene is one of the genes responsible for the production of beta-carotene in the endosperm and leaves of maize. We have cloned a Robertson's Mutator-tagged allele of the y1 gene (y1-mum) by using a Mu3 element as a hybridization probe. We substantiate that the cloned sequence is a portion of the y1 gene by molecular analyses of a revertant of a putative Mutator-induced y1 allele and the incidence of insertions within the cloned y1 sequence from several independently derived Mutator-induced y1 mutant stocks. The y1-mum sequence was used to isolate the standard Y1 allele, which conditions the presence of beta-carotene in the endosperm of the maize kernel.
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