Cis-regulatory elements for pistil specific expression in S-RNase promoter region of Japanese pear (Pyrus pyrifolia Nakai)
2002
Dissanayake, D.M.R.K.K. | Norioka, S. | Norioka, N. | Takasaki, T. | Nakanishi, T.
The pistil-specific and temporal expression of Japanese pear S-RNase gene is regulated by S-RNase promoter region. To identify the cis-regulatory elements on the S-RNase promoter of Japanese pear, we analysed GUS activities in transgenic tobacco plants carrying 9 sequential deletions of S3-RNase promoter fused to the -glucuronidase (GUS) gene. GUS activity was detected quantitatively in the pistils from the transgenic plants carrying the promoter deleted from -747 to -310. GUS activity was localized in the stigmatic secretory zone, and the transmitting tissues in the pistils but not in other tissues; pollen, anthers, petals, sepals, leaves and roots. GUS activities in the pistils increased steadily from 4 days before flowering. No transgenic pistils with deletions carrying -255 or shorter promoters displayed GUS activity, indicating that the critical cis-regulatory elements for pistil specific expression were present between -310 and -255 of S3-RNase promoter region. Furthermore, deletions from -552 to -356 led to the drastic decrease of GUS activity in the transgenic pistil, which assumes that enhancer-like element for the high level of expression in pistils are present within this region.
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