Sample size for collecting seeds in germplasm conservation: the case of the Lima bean (Phaseolus lunatus L.)
1998
Bi, I.Z. | Maquet, A. | Degreef, J. | Wathelet, B. | Baudoin, J.P.
The design of optimum sampling strategies integrating criteria of efficiency relevant to multilocus models and many target populations has been investigated with respect to the number of plants and the number of seeds per plant to be sampled for a Lima bean (Phaseolus lunatus L.) gene pool. This study, using five populations and six polymorphic enzyme loci, shows that the number of plants rather than the number of seeds collected per plant primarily determines the success of seed sampling, suggesting that plant number plays an essential part in maintaining the allelic multiplicity of predominantly selfing species like Lima bean. According to the results, it appears that among Lima bean populations an efficient sampling procedure is achieved by collecting 1-4 seeds from 200 to 300 plants. These sample sizes will retain 8-10 alleles, regardless of their frequencies. When we consider polymorphism at the 5% level, it is expected that sampling 10-80 plants will collect combinations of 4-8 alleles. Based on data from genetic and demographic studies, we suggest an efficient sampling scheme for Lima bean germplasm at both population and geographical levels.
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