Multiple Shoot Induction from Cotyledonary Node Explants of Terminalia chebula
2003
Shyamkumar, B. | Anjaneyulu, C. | Giri, C.C.
A protocol for multiple shoot induction from cotyledonary node explants of Terminalia chebula Retz. has been developed. Germination frequency of embryos (up to 100 %) was obtained on Murashige and Skoog (MS) medium supplemented with 0.5 mg dm⁻³ gibberellic acid (GA₃). Maximum number of shoots (6.4 shoots per cotyledonary node) was obtained on half-strength MS + 0.3 mg dm⁻³ GA₃+ 1.0 mg dm⁻³ indole-3-butyric acid (IBA) + 10.0 mg dm⁻³ benzylaminopurine (BAP) after 4 weeks of culture. When the cotyledonary nodes along with the axillary shoot buds were allowed to grow in the same medium upto 19.2 shoots were obtained after 8 – 9 weeks. Best rooting (100 %, 5.5 roots per shoot) was observed when shoots were excised and transferred to half-strength MS medium containing 1.0 mg dm⁻³ IBA + 1 % mannitol and 1.5 % sucrose. Survival of rooted plants in vivo was low (35 – 40 %) when they were directly transferred to soil in glasshouse. However, transfer to soil with MS nutrients and 1.0 mg dm⁻³ IBA in culture room for a minimum duration of 2 weeks increased the survival percentage of plants to 100 %.
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