Xylitol production from corn fibre hydrolysates by a two-stage fermentation process
2000
Leathers, T.D. | Dien, B.S.
A two-stage fermentation process was developed for production of xylitol from corn fibre hydrolysates by Pichiaguilliermondii NRRL Y-12723. Initial studies using purified sugars showed that P.guilliermondii was able to produce xylitol and arabitol from a mixture of xylose and arabinose; however, when cells were grown on mixtures of glucose, xylose and arabinose in ratios similar to those found in corn fibre hydrolysates, glucose was utilized preferentially, while the pentoses were metabolized slowly resulting in low yields of xylitol and arabitol. To overcome this problem, a two-stage fermentation scheme was developed in which cells were removed from mixed sugar cultures following glucose consumption and replaced with cells grown on xylose alone. These cells could then ferment the mixture of xylose and arabinose to xylitol and arabitol. After treatment with a mixed-bed deionization resin, dilute acid corn fibre hydrolysates were shown to be suitable substrates for the two-stage process.
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