Metagenomic data of free cyanide and thiocyanate degrading bacterial communities
2017
Mekuto, Lukhanyo | Ntwampe, Seteno K.O. | Mudumbi, John B.N. | Akinpelu, Enoch A. | Mewa-Ngongang, Maxwell
The data presented in this article contains the bacterial community structure of the free cyanide (CN⁻) and thiocyanate (SCN⁻) degrading organisms that were isolated from electroplating wastewater and synthetic SCN⁻ containing wastewater. PCR amplification of the 16S rRNA V1-V3 regions was undertaken using the 27F and 518R oligonucleotide primers following the metacommunity DNA extraction procedure. The PCR amplicons were processed using the illumina® reaction kits as per manufacturer׳s instruction and sequenced using the illumina® MiSeq-2000, using the MiSeq V3 kit. The data was processed using bioinformatics tools such as QIIME and the raw sequence files are available via NCBI׳s Sequence Read Archive (SRA) database.
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