Reaction of winter oilseed rape callus to different concentrations of elicitors: pectinase or chitosan
2003
Płażek, A. | Hura, K. | Żur, I.
The aim of the presented methodical experiments was 1) the evaluation if callus of winter oilseed rape (Brassica napus var. oleifera L.) initiates a defence reaction to fungal elicitors: pectinase (polygalacturonase) or chitosan, and 2) the choice of the elicitor doses, which evoke the strongest tissue reaction. The results obtained will be used in the next experiments relating the studies of pathogenesis mechanisms proceeding in rape plants infected by necrotrophic fungi. The defence response was estimated on the basis of changes in electrolyte leakage from cells, metabolic efficiency, phenolic content and catalase activity. In the experiment pectinase was used at concentration of 3, 8, 16, 133 and 166 µl per 1 cm³ of culture medium while chitosan at: 25, 50, 75 and 100 µg·cm⁻³. Both elicitors increased cell membrane permeability: pectinase at the doses equal or greater to 16 µl·cm⁻³ while chitosan of 25 µg·cm⁻³. The greatest metabolic inefficiency was observed in calli elicited with 16 µl·cm⁻³ pectinase and with chitosan of 100 µg·cm⁻³. The decrease in phenolic content was noted under influence of most doses of both elicitors. The highest catalase activity was evoked by pectinase of 8 µl·cm⁻³ and chitosan of 75 and 100 µg·cm⁻³. The results indicated that 8–16 µl·cm⁻³ of pectinase and 100 µg·cm⁻³ of chitosan caused the strongest defence reaction of oilseed rape tissue.
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