Assessment of β-carotene content, cell physiology and morphology of the yellow yeast Rhodotorula glutinis mutant 400A15 using flow cytometry
2013
Cutzu, Raffaela | Clemente, Ana | Reis, Alberto | Nobre, Beatriz | Mannazzu, Ilaria | Roseiro, José | Lopes da Silva, Teresa
Flow cytometry was used to assess β-carotene content, cell membrane permeability, cell size and granularity in Rhodotorula glutinis mutant 400A15 grown under different oxygen transfer coefficients (kLa) and carbon to nitrogen ratios (C/N). A Doehlert distribution was used in order to select the best conditions that induced the highest carotenoids production. The highest β-carotene content (0.79 mg g⁻¹DCW) at the lowest kLa and C/N (5 × 10⁻³ s⁻¹and 11.3 respectively). Under these conditions, the biomass concentration attained 18.60 g L⁻¹. The highest ratio of cells with permeabilised membranes (2.6 %), and the highest cell size and granularity were also obtained under these conditions. It was observed that C/N showed a stronger influence than the kLa on the measured cell parameters.
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