Gomphoneis tegelensis sp. nov. (Bacillariophyceae): a morphological and molecular investigation based on selected single cells
2018
Skibbe, Oliver | Zimmermann, Jonas | Kusber, Wolf-Henning | Abarca, Nélida | Buczkó, Krisztina | Jahn, Regine
Colonies of a new, stalked diatom species Gomphoneis tegelensis sp. nov. were sampled in September 2017 from Lake Tegel (Berlin, Germany) where this large species was first observed in 2009. Cultivation was attempted several times without success. A single-cell DNA extraction method was therefore tried to yield molecular and morphological data from non-cultured specimens. Single cells were selectively picked off their stalks and their cell walls broken to release the DNA, as PCR and sequencing of intact cells provided no results. To improve yield, the DNA of several cells was pooled after PCR. The remaining intact cells were used for ultrastructural studies under SEM. The closely related species Gomphoneis transylvanica is typified as a basis for proper taxon identification and for comparison with the newly described species. Gomphoneis tegelensis differs from G. transylvanica and Gomphoneis ohridana mainly in its striation pattern and the number of rows of areolae in each stria. Molecular data confirm that G. tegelensis belongs to the Cymbellales, but it does not belong to the Gomphonemataceae.
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