Development of a new fluorescence immunochromatography strip for detection of chloramphenicol residues in chicken muscles
2013
Bai, Zhihong | Luo, Yunbo | Xu, Wentao | Gao, Huafang | Han, Pei | Liu, Tao | Wang, Hui | Chen, Ailiang | Huang, Kunlun
BACKGROUND: Chloramphenicol (CAP), an antimicrobial drug that is widely used in animal feed, would have a negative effect on human health due to its low elimination rate and relatively high residue in animal food. It is important to develop a rapid and economic method to determine CAP in animal food to ensure that human health is not affected. RESULTS: A new fluorescence immunochromatography strip was developed and established for the detection of CAP residue in chicken muscles for the first time. A CAP–bovine serum albumin conjugate, monoclonal antibody and polyclonal antibody against CAP were applied to constitute a fluorescence immunochromatography strip. The fluorescence intensity was detected by a charge‐coupled device scanner and transformed to a digital value. The CAP linearity working range was from 0.1 ng mL⁻¹ to 20 ng mL⁻¹ with a limit of detection of 0.1 ng mL⁻¹ within 10 min. The performance of the strip assay was compared with a commercial ELISA kit and the correlation coefficient was 0.99, which indicated that the new strip assay had a good quantification ability for CAP. CONCLUSION: The fluorescence immunochromatography strip was successfully applied to the detection of CAP residues in chicken samples. To our knowledge, it is the first report regarding the development of a fluorescence immunochromatography method for screening CAP in animal samples. © 2013 Society of Chemical Industry
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