Developing Midstearic Acid Sunflower Lines from a High Stearic Acid Mutant
2004
Pérez-Vich, Begoña | Muñoz-Ruz, Juan | Fernández-Martínez, José M.
Sunflower (Helianthus annuus L.) genotypes with increased stearic acid (C18:0) content in their seed oil may be useful for food and industrial applications. The objective of this study was to isolate and characterize mid-stearic acid sunflower lines homozygous recessive for single genes from the high stearic acid mutant CAS-3 (250 g kg⁻¹). Crosses between CAS-3 (genotype es1es1es2es2), and the inbred line HA 89, with standard low stearic acid levels (50 g kg⁻¹; Es1Es1Es2Es2), were made to obtain F₃ families segregating for either the Es1 or Es2 loci. From these families, F₃ half-seeds with putative genotypes es1es1Es2Es2 (188 g kg⁻¹) and Es1Es1es2es2 (90 g kg⁻¹) were selected. F₇–lines homozygous for es1 or es2 were developed. These lines were named CAS-19 (es1es1Es2Es2) and CAS-20 (Es1Es1es2es2) and showed mid-stearic acid levels of 168 g kg⁻¹ (CAS-19) and 83 g kg⁻¹ (CAS-20). The fatty acid content was evaluated in all generations by gas-liquid chromatography. The genetic composition of CAS-19 and CAS-20 was verified by evaluating progenies from crosses between both mid-stearic acid lines, and from crosses between the mid-stearic acid lines and HA 89. The new Es1Es1es2es2 and es1es1Es2Es2 genotypes expressing mid-stearic acid levels represent a further advance for the development of sunflower lines for specific edible purposes, and constitute a unique source for agronomic and genetic studies on single alleles controlling increased stearic acid content in sunflower.
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