Increased crocin production and induction frequency of stigma-like-structure from floral organs of Crocus sativus by precursor feeding
2003
Zeng, Y. | Yan, F. | Tang, L. | Chen, F.
The effect of different additives (sodium acetate, serine, glycine, PVP and activated charcoal) on stigma-like-structure (SLS) induction frequency and crocin production from floral organs of Crocus sativus L. was studied. Sodium acetate, a potential precursor of crocin biosynthesis, increased induction frequency of petal-originated SLS from 16.0 to 32.7%. Moreover, crocin content in SLS induced on medium supplemented with sodium acetate was increased to 6.00% from 2.21%. PVP prevented browning of explants and slightly increased induction frequency of SLS from petals. The crocin content of SLS was also increased to 5.22% by adding PVP. Although activated charcoal could efficiently reduce browning of explants, dedifferentiation of all explants was inhibited. No obvious stimulation effect had been observed with the addition of serine and glycine.
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