The Influence of Nitroxyl on Escherichia coli Cells Grown under Carbonyl Stress Conditions
2022
Nasybullina, E. I. | Pugachenko, I. S. | Kosmachevskaya, O. V. | Topunov, A. F.
Carbonyl stress is an increase in the amount of monosaccharides and active dicarbonyl compounds (glyoxal and methylglyoxal (MG)), which leads to raising the rate of formation of advanced glycation end-products (AGEs). MG added to an Escherichia coli culture inhibited the growth of bacteria, while the number of fluorescent proteins-associated AGEs increased. The effect of nitroxyl (HNO) on cells depended on the level of aeration of the bacterial culture. Nitroxyl decreased the toxic effect of MG on the bacterial culture, which was expressed in an increase of cell viability, assessed by the MTT test, and in the decrease of the autofluorescence of nonenzymatic glycation products associated with proteins. Under lowered aeration conditions, the cytoprotective effect of a nitroxyl donor, Piloty’s acid, was more obvious. The cytoprotective effect of HNO at carbonyl stress conditions may be associated with its antioxidant and antiglycemic effects. The results of the study are important for understanding the mechanisms of the protective and regulatory action of HNO in cells.
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