Isolation and characterization of ethanol-producing yeasts from fruits and tree barks
2008
Rao, R.S. | Bhadra, B. | Shivaji, S.
Isolation and identification of yeasts converting xylose to ethanol. A total of 374 yeasts were isolated from a variety of rotten fruits and barks of trees. Out of these, 27 yeast strains were able to assimilate xylose and produce 0·12-0·38 g of ethanol per gram of xylose. Based on phylogenetic analysis of D1/D2 domain sequence of LSU (Large Subunit) rRNA gene and phenotypic characteristics the ethanol-producing strains were identified as member(s) of the genera Pichia, Candida, Kluyveromyces, Issatchenkia, Zygosacchraomyces, Clavispora, Debaryomyces, Metschnikowia, Rhodotorula and Cryptococcus. Yeast strains producing ethanol from xylose have been isolated from a variety of rotten fruits and barks of trees and identified. Environmental isolates of yeasts which could convert xylose to ethanol could form the basis for bio-fuel production and proper utilization of xylan rich agricultural and forest wastes.
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