Purification of eleutherosides by macroporous resin and the active fractions of anti-inflammatory and antioxidant activity from Acanthopanax senticosus extract
2013
Liu, Shun | Chen, Yan | Gu, Lijuan | Li, Yongxia | Wang, Bingyi | Hao, Jie | Kitanaka, Susumu | Li, Heran
Eleutherosides are important active ingredients of Acanthopanax senticosus, the method for purification of eleutherosides has attracted much attention. In this article, a method for purification of eleutherosides by macroporous resin and the anti-inflammatory and antioxidant activity of elution fractions from Acanthopanax senticosus extract are investigated. Choosing syringin and eleutheroside E as an indication of eleutherosides, the adsorption characteristics and elution parameters of eleutherosides were investigated from Acanthopanax senticosus extract by various types of macroporous resins. The results show HPD100 provides a much higher adsorption and desorption capacity and the retention rates of syringin and eleutheroside E in the product were 95.43% and 94.96%, respectively. Moreover, the 10–80% ethanol elution fractions were collected from the purification process. We examined the anti-inflammatory activity by evaluating Nitric Oxide (NO) production and the antioxidant activities of eight ethanol elution fractions. As a result, 10–80% ethanol elution fractions showed strong concentration-dependent NO production inhibitory activities and antioxidant activities. The 30–50% ethanol elution fractions are shown to be more active than other fractions for antioxidant and anti-inflammatory effects. In addition, no cytotoxicity was observed from the three most potent fractions.
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