Studies on the in vitro regenerative competence of aerial roots of two horticultural important Cymbidium species
2012
Deb, Chitta Ranjan | Pongener, Aolemla
In the present study aerial roots were successfully used as explants source for in vitro propagation of Cymbidium aloifolium and Cymbidium iridioides. Aerial roots of ~5–6 week old from axenic cultures were cultured on MS medium adjuncts with different additives. In C. aloifolium within 20 days of culture ~60% of explants responded positively on MS medium containing sucrose (3%, w/v) and Kn (3 μM) and formed PLBs. While in C. iridioides ~50% root explants responded positively on medium enriched with sucrose (3%), AC (0.1%) and IAA (3 μM) after 40 days of culture. The shoot buds/PLBs converted into plantlets when maintained on regeneration medium. Of the three basal media tested, MS medium supported optimum regeneration and culture proliferation in both the species. In C. aloifolium ~12 shoot buds developed on medium nourished with sucrose (3%) and BA (3 μM) but in C. iridioides optimum regeneration was achieved when medium supplemented with sucrose (3%), CW (15%), CH (100 mg L−1) and ~20 shoot buds formed per subculture. The well rooted plantlets were acclimatized for 3–4 week in 1/10th MS salt solution containing sucrose (1%), charcoal pieces, brick pieces and chopped mosses as support under normal laboratory conditions. The hardened plants were transferred to potting mix where 80 and 75% of transplants survived in C. aloifolium and C. iridioides respectively after 2 months of transfer.
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