Optimization of culture medium for the production of phenyllactic acid by Lactobacillus sp. SK007
2009
Mu, Wanmeng | Chen, Chao | Li, Xingfeng | Zhang, Tao | Jiang, Bo
Optimization of medium components for the production of PLA by the strain Lactobacillus sp. SK007, which was isolated from Chinese traditional pickles, was carried out using response surface methodology (RSM) based on a uniform design and a central composite rotatable design (CCRD). The final concentration of the optimized medium was 30gL⁻¹ glucose, 5gL⁻¹ phenylpyruvic acid (PPA), 47gL⁻¹ corn steep liquor, 3gL⁻¹ K₂HPO₄, 3gL⁻¹ CH₃COONa, 30gL⁻¹ yeast powder and 3mLL⁻¹ Tween-80. A maximum PLA yield of 2.30gL⁻¹ was achieved, which was significantly higher than previously reported.
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