Hydrogen sulfide-induced chilling resistance in peach fruit is performed via sustaining the homeostasis of ROS and RNS
2023
Zhao, Yaqin | Zhao, Liangyi | Hu, Shunqing | Hou, Yuanyuan | Wang, Jingda | Zheng, Yonghua | Jin, Peng
Hydrogen sulfide (H₂S) application impacts on endogenous H₂S, reactive oxygen species (ROS), and reactive nitrogen species (RNS) in cold-stored peach fruit were investigated. 20 μL L⁻¹ H₂S (NaHS as the donor) efficiently retarded the quality deterioration arising from chilling injury (CI), triggering endogenous H₂S production, while enhancing antioxidant systems and ROS generation (NADPH oxidative enzyme). H₂S promoted nitric oxide (NO) correlated with the S-nitrosoglutathione reductase (GSNOR)-mediated GSNO reduction, while suppressing the peroxynitrite anion content. As the pivotal coenzyme of ROS and RNS, nicotinamide adenine dinucleotide phosphate (NADPH) levels were elevated by H₂S during late-stage storage via the tricarboxylic acid cycle, where reduced NADP-isocitrate dehydrogenase (NADP-ICDH) activity and gene expression. Structural analysis of peach NADP-ICDH (UniProtKB M5WXP5) deduced that Cys79 and Tyr396 are the likeliest targets for S-nitrosylation and nitration, respectively. These results indicate that H₂S counteracts the disorders of ROS and RNS to ameliorate CI of peach fruit.
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