Effect of pH on the sorption of dissolved organic carbon derived from six tree species in forest soils

Dissolved organic carbon (DOC) is an important component of the total carbon (C) pool in temperate forest soils. The ability of forest soils to sorb DOC is an essential feature in the context of ecosystem changes (such as climate change) and the resulting changes in the C cycle. The aim of this study was to investigate the ability of forest soils with different pH levels to sorb DOC from litter of different forest tree species. To accomplish this, we conducted two laboratory experiments in which the soil differed in the depth from which they were sampled (topsoil vs. subsoil) and the initial DOC content. We modified the soil pH to obtain a range covering most soils in temperate forests (approximately 3.5–8). Soil samples were leached with extracts of similar DOC content obtained from the litter of six main forest-forming tree species in temperate zone. Increased DOC sorption was observed at approximate soil pH levels of 3.5 and 7.5, with the highest sorption capacity occurring at low pH. The DOC sorption capacity was decreasing with consecutive leaching with litter extract. The capacity of DOC sorption also depended on the litter species. In addition, the initial concentration of C in soil had no significant relationship with sorption capacity. Litter species were related to the sorption of released DOC, which was probably linked to the Al content in the litter. The increased DOC sorption at low pH was likely because of dissolved organic matter protonation (saturation of functional groups with H⁺ ions resulting in proton bridging and van der Waals forces), while Ca²⁺ ions causing cation bridging, flocculation or non-columbic interactions were likely responsible for the increased sorption at high pH. Our research shed light on the significant effects of soil pH on the stabilization of DOC, as well as the important role and positive influence of Al on DOC sorption by forest soils. Our findings provide new, significant insight by presenting that litter-derived DOC sorption is dependent both on soil pH and litter chemical composition.