Isolation of eriocitrin (eriodictyol 7-O-rutinoside) as an arachidonate lipoxygenase inhibitor from Lumie fruit (Citrus lumia) and its distribution in Citrus species
2007
Nogata, Y. | Ohta, H. | Ishii, T. | Sekiya, K.
An inhibitory compound acting against rat platelet 12-lipoxygenase was isolated from the peel of Lumie fruit (Citrus lumia) by activity-guided separation. It was identified as eriocitrin (eriodictyol 7-O-rutinoside) by spectroscopic analyses. Eriocitrin inhibited 5-lipoxygenase (IC₅₀29.1 micromol L⁻¹) from rat peritoneal polymorphonuclear leukocytes in addition to 12-lipoxygenase (IC₅₀22.3 micromol L⁻¹). Its aglycone, eriodictyol (5,7,3', 4'-tetrahydroxyflavanone), was a much more potent inhibitor of both 12-lipoxygenase (IC₅₀0.07 micromol L⁻¹) and 5-lipoxygenase (IC₅₀0.20 micromol L⁻¹). It also inhibited the production of leukotriene B₄ in intact peritoneal polymorphonuclear leukocytes stimulated with calcium ionophore A23187 (IC₅₀12.7 micromol L⁻¹). The distribution of eriocitrin in 39 citrus fruits was investigated by high-performance liquid chromatography analysis. Lumie, eureka lemon (Citrus limon), Sambokan (Citrus sulcata), Sudachi (Citrus sudachi) and Koji (Citrus leiocarpa) fruits were found to contain high levels of eriocitrin in both peel and juice vesicles.
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