Increasing glutathione formation by functional expression of the γ-glutamylcysteine synthetase gene in Saccharomyces cerevisiae
2004
Fan, X. | He, X. | Guo, X. | Qu, N. | Wang, C. | Zhang, B.
A recombinant plasmid, pGMF, containing a γ-glutamylcysteine synthetase gene (GSH-I) from Saccharomyces cerevisiae, was constructed with a copper-resistance gene as the selection marker and was introduced into S. cerevisiae YSF-31. The glutathione content of the recombinant strain was 1.5-fold (13.1 mg g dry cells-1) of that in the host strain.
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