Simple derivatization of aldehydes with d-cysteine and their determination in beverages by liquid chromatography–tandem mass spectrometry
2011
Kim, Hyun-Ji | Shin, Ho-Sang
We describe a simple derivatization method to determine aldehydes. This method is based on derivatization with d-cysteine and consecutive liquid chromatography–tandem mass spectrometry (LC–MS/MS). The optimum derivatization conditions of aldehydes with d-cysteine were 10min at 50°C and pH 7.0. The formed alkyl thiazolidine-4-carboxylic acid derivatives were directly injected in LC–MS/MS. In the established condition, the method was used to detect eight aldehydes in beverages. The limit of detection (LOD) and limit of quantification (LOQ) of the aldehydes were 0.2–1.9μgL⁻¹ and 0.7–6.0μgL⁻¹ and the relative standard deviation was less than 2.0% at concentrations of 0.1mgL⁻¹ and 1.0mgL⁻¹ with the exception of octanal. All the beverage samples had detectable levels of methanal (0.033–0.145mgL⁻¹), ethanal (0.085–2.12mgL⁻¹), propanal (ND to 0.250mgL⁻¹), butanal (ND to 0.003mgL⁻¹), pentanal (ND to 0.471mgL⁻¹), hexanal (ND to 0.805mgL⁻¹), heptanal (0.019–3.91mgL⁻¹) and octanal (0.029–0.118mgL⁻¹).
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