Partial purification and reconstitution of the alpha-ketoglutarate carrier from corn (Zea mays L.) mitochondria
1991
Genchi, G. | De Santis, A. | Ponzone, C. | Palmieri, F.
The alpha-ketoglutarate carrier from corn shoot mitochondria (Zea mays L., B 73) was solubilized in Triton X-114 and partially purified by chromatography on hydroxyapatite and celite in the presence of cardiolipin. On SDS-gel electrophoresis, the hydroxyapatite/celite eluate showed various protein bands between 12 and 70 kilodaltons. When reconstituted into liposomes, the alpha-ketoglutarate transport protein catalyzed a phthalonate-sensitive alpha-ketoglutarate/alpha-ketoglutarate exchange. The protein was purified 60-fold with a recovery of 88% with respect to the mitochondrial extract. The protein yield was 0.6%. The properties of the reconstituted carrier, i.e. requirement for a counter-anion, substrate specificity, and inhibitor sensitivity, were similar to those of the alpha-ketoglutarate transport system as characterized in plant and animal mitochondria.
Mostrar más [+] Menos [-]Palabras clave de AGROVOC
Información bibliográfica
Este registro bibliográfico ha sido proporcionado por National Agricultural Library