Two-Dimensional High Performance Liquid Chromatography-Mass Spectrometry for Phosphatidylcholine Analysis in Egg Yolk
2015
Walczak, Justyna | Bocian, Szymon | Buszewski, Boguslaw
Phospholipids are one of the classes of lipids, which are a major component of all cell membranes. Phosphatidylcholine (PC) is the main phospholipid present in egg yolk. The phospholipids extract from enriched egg yolk was separated using the two-dimensional high performance liquid chromatography in off-line system coupled with electrospray ionization tandem mass spectrometry (off-line HPLC-ESI-MS/MS). In the first dimension, a preparative C18 column was used, and on the second dimension, a separation was performed on N,O-dialkylphosphoramidate bonded silica column. In the analyzed egg yolk, 15 different phosphatidylcholines was found. From among the identified fatty acid of PC, a large part constitutes docosahexaenoic acid (ω-3, 22:6, DHA) and linoleic acid (ω-6, 18:2, LA).
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