Determination of multi-walled carbon nanotube bioaccumulation in earthworms measured by a microwave-based detection technique
2013
Li, Shibin | Irin, Fahmida | Atore, Francis O. | Green, Micah J. | Cañas-Carrell, Jaclyn E.
Reliable quantification techniques for carbon nanotubes (CNTs) are limited. In this study, a new procedure was developed for quantifying multi-walled carbon nanotubes (MWNTs) in earthworms (Eisenia fetida) based on freeze drying and microwave-induced heating. Specifically, earthworms were first processed into a powder by freeze drying. Then, samples were measured by utilizing 10s exposure to 30W microwave power. This method showed the potential to quantitatively measure MWNTs in earthworms at low concentrations (~0.1μg in 20mg of earthworm). Also, a simple MWNT bioaccumulation study in earthworms indicated a low bioaccumulation factor of 0.015±0.004. With an appropriate sample processing method and instrumental parameters (power and exposure time), this technique has the potential to quantify MWNTs in a variety of sample types (plants, earthworms, human blood, etc.).
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