Phytotoxicity of atrazine combined with cadmium on photosynthetic apparatus of the emergent plant species Iris pseudacorus

The combined pollution, instead of single pollution, has become a widespread contamination phenomenon in aquatic environment. However, little information is now available about the joint effects of the combined pollution, especially co-existed pesticides and heavy metals, on aquatic plants. In the present study, using continuous excitation chlorophyll fluorescence parameters and the OJIP transient, comparisons of herbicide atrazine (ATZ) phytotoxicity on Iris pseudacorus between in the presence and absence of cadmium (Cd) were evaluated over an exposure period of three weeks under laboratory conditions. Results showed that both ATZ and Cd were toxic to I. pseudacorus. The ratio Fᵥ/Fₒ, specific electron transport energy (ET₀/RC), and photochemistry efficiency (PIₐbₛ and PIₜₒₜₐₗ) of this emergent plant species at individual ATZ and Cd concentrations were significantly lower than those of the control. ATZ mainly inhibited electron transport beyond QA at PSII acceptor side as indicated by the sharp rise of the J-step level of fluorescence rise kinetics. A pronounced K-step and the loss of I-step due to the damage on the OEC and PSI also occurred when ATZ was at or above 1.0 mg·L⁻¹. In comparison to ATZ alone, ATZ combined with Cd resulted in a lower amplitude rise in J-step with apparent J-I and I-P phases; and significantly lower Fₒ with higher Fᵥ/Fₒ, as well as greater ET₀/RC with higher values of PIₐbₛ and PIₜₒₜₐₗ. However, the adverse influences of ATZ combined with Cd on the above indicators were still significant as compared with the control. Therefore, the coexistence of Cd alleviated the individual phytotoxicities of ATZ, whereas combined pollution of ATZ and Cd still induced the decline in photosynthetic performance of I. pseudacorus, and its potential ecological impacts on the aquatic vegetation cannot be ignored. Our findings offer a better understanding of the joint effects of the pesticide and heavy metal on non-target aquatic plants, and provided valuable insights into the interaction of these pollutants in aquatic environment.