Comparison of our techniques for measuring growth of Tetrahymena pyriformis W with rat per bioassays in assessing protein quality
1979
Wang, Y.Y.D. | Miller, J. | Beuchat, L.R.
Extract: Four techniques for measuring growth of Tetrahymena pyriformis W (TpW), namely, direct microscopic count (DMC), tetrazolium dye-reduction (TPTZ), oxygen uptake (0(sub2)-UT), and ATP bioluminescence (ATP-biol.), were compared with rat protein efficiency ratio (PER) assays for assessing protein quality. Relative nutritive value (RNV) of the test material was compared to a casein standard by slope ratio assay and by use of one concentration of nitrogen in the growth medium of TpW. It was concluded that TPTZ, 0(sub2)-UT, or ATP-biol. could be used to replace the tedious and time-consuming DMC in measuring growth of TpW for protein quality assay.
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